| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,365,057 | 0 | A | T | 65.1% | 69.4 / 69.3 | 66 | pseudogene (18/480 nt) pseudogene (18/1745 nt) | yhcE yhcE | ECK3207:JW3184:b3217; hypothetical protein, N‑ter fragment ECK3207:JW3184+JW3187:b4569; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (23/0); new base T (0/43); total (23/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.87e-18 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCAT > W3110S.gb/3365034‑3365119 | gTGCCAATGACACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:371183/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCTTGGCCGCTCAGGCaga < 1:1228946/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCATGCaga < 1:1580099/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCtga < 1:326051/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2217631/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:600986/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:588899/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:566323/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2069604/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2146331/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2165213/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:50880/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:481028/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2217266/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:646407/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:401262/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2275849/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2294453/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2327520/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2366485/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2458342/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:14600/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1066164/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1116766/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:999886/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:983557/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1265667/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1297576/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1305421/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1319973/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:915994/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1864889/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1475240/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1495315/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1546771/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:850302/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:655035/65‑1 (MQ=255)gTGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1737330/65‑1 (MQ=255) tGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:2042064/64‑1 (MQ=255) tGCCAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1764272/64‑1 (MQ=255) ccAATGAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:1082701/62‑1 (MQ=255) gAAACGGATAATAACTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:558768/57‑1 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:1993120/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:977650/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:1348464/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:852195/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:1611809/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:83310/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:780360/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:1652955/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:1785796/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:631771/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:61409/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:1174054/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:2168533/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:2193331/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:430704/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:2251771/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:2367779/1‑63 (MQ=255) cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtc > 1:2390172/1‑63 (MQ=255) taaCTGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga < 1:368368/46‑1 (MQ=255) cAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAgtgt > 1:503084/1‑47 (MQ=255) cAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCAt > 1:117066/1‑64 (MQ=255) cAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCAt > 1:308688/1‑64 (MQ=255) cAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCAt > 1:1939886/1‑64 (MQ=255) cAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGACTGTaa > 1:200517/1‑49 (MQ=255) | GTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCAT > W3110S.gb/3365034‑3365119 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |