| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,246,122 | 1 | . | A | 66.7% | 44.7 / 22.3 | 21 | intergenic (‑276/+96) | xylE/malG | D‑xylose transporter/maltose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/7); new base A (0/14); total (0/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACCGGCGAACGATTCAGATTGCAGACGAAAG‑AAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTA > W3110S.gb/4246091‑4246176 | aaCCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATaa < 1:231420/64‑1 (MQ=255) cGGCGAACGATTCAGATTGCAGACGAAAG‑AAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGt < 1:136101/64‑1 (MQ=255) cGGCGAACGATTCAGATTGCAGACGAAAG‑AAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGt < 1:146599/64‑1 (MQ=255) cGGCGAACGATTCAGATTGCAGACGAAAG‑AAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGt < 1:1579440/64‑1 (MQ=255) cGGCGAACGATTCAGATTGCAGACGAAAG‑AAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGt < 1:1582299/64‑1 (MQ=255) cGGCGAACGATTCAGATTGCAGACGAAAG‑AAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGt < 1:1624744/64‑1 (MQ=255) cGGCGAACGATTCAGATTGCAGACGAAAG‑AAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGt < 1:2388645/64‑1 (MQ=255) ttCAGATTGCAGACGAAAG‑AAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGt < 1:2492277/54‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCTTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:1704577/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:1934449/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:998831/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:639183/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:533488/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:529659/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:469318/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:2345670/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:2300976/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:1032285/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:1897397/64‑1 (MQ=255) aGACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:1283119/64‑1 (MQ=255) gACGAAAGAAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTa < 1:1446027/63‑1 (MQ=255) | AACCGGCGAACGATTCAGATTGCAGACGAAAG‑AAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTA > W3110S.gb/4246091‑4246176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |