| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,413,370 | 0 | A | T | 73.0% | 61.8 / 25.2 | 37 | N679Y (AAT→TAT) | rnr | exoribonuclease R, RNase R |
| Reads supporting (aligned to +/- strand): ref base A (10/0); new base T (0/27); total (10/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.87e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCA > W3110S.gb/4413308‑4413428 | gcttCTTCGTCCGTCTGGACGTCTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1929809/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1745916/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:273406/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1413344/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1452678/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1497554/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:149860/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:806186/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1555119/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1594178/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:2441442/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1491291/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1750044/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:2415082/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1777877/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1826760/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:698909/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1850654/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:2093660/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:2079962/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1181819/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1054302/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1096647/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:5821/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1112090/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1383525/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:497890/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:464571/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:411005/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1340869/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:134688/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:407181/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:610953/64‑1 (MQ=255)gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATCGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1559777/64‑1 (MQ=255)gcttCTTCGTCCGGCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:105745/64‑1 (MQ=255)gcttCTTCGTCCGGCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1074402/64‑1 (MQ=255) cttcttCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:2489959/63‑1 (MQ=255) cttcttCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1836340/63‑1 (MQ=255) ggACGACTTGTTCTTTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1056276/48‑1 (MQ=255) cgacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1877145/45‑1 (MQ=255) gTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACaa < 1:1171478/39‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAATGCCTGATGGGGGa < 1:42337/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:287412/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:2112621/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:672385/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:2142828/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:2235061/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:591523/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:2467459/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1025523/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1228645/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1234156/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1188834/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1352070/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1366145/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1111165/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1402463/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:204131/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1638602/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:788328/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1758204/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1805216/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1817054/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:702337/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1831980/65‑1 (MQ=255) cATGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:191693/65‑1 (MQ=255) aTGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:636034/64‑1 (MQ=255) tGTCTCTTCGCTGGACTATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGa < 1:1319353/63‑1 (MQ=255) cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATcc > 1:1382007/1‑57 (MQ=255) cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCa > 1:983848/1‑65 (MQ=255) cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCa > 1:307032/1‑65 (MQ=255) cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCa > 1:2538284/1‑65 (MQ=255) cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCa > 1:2118392/1‑65 (MQ=255) cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCa > 1:183456/1‑65 (MQ=255) cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCa > 1:1822863/1‑65 (MQ=255) cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCa > 1:1639480/1‑65 (MQ=255) cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCa > 1:1503819/1‑65 (MQ=255) cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCa > 1:1481397/1‑65 (MQ=255) | GCTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCA > W3110S.gb/4413308‑4413428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |