Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	75,677	A→C	F541L (TTT→TTG)	sgrR ←	DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	75,677	0	A	C	100.0%	56.5 / NA	16	F541L (TTT→TTG)	sgrR	DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/2);  total (14/2)

TATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCG  >  W3110S.gb/75631‑75713                                               |                                     tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:1219735/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:1345005/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:154945/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:1657452/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:1683306/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:1742849/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:1870863/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:191475/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:1921220/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:2272073/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:606371/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:620698/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:757918/1‑64 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg                     >  1:923631/1‑64 (MQ=255)                   gATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcg  <  1:1328090/65‑1 (MQ=255)                   gATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcg  <  1:1599013/65‑1 (MQ=255)                                               |                                     TATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCG  >  W3110S.gb/75631‑75713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑