Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	505,179	C→T	L348L (CTG→TTG)	ushA →	bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	505,179	0	C	T	100.0%	138.9 / NA	40	L348L (CTG→TTG)	ushA	bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/0);  total (40/0)

CTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAG  >  W3110S.gb/505144‑505214                                    |                                    cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:650324/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:329271/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:1786839/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:186358/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:2085508/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:1665176/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:1602477/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:673347/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:749215/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:2244471/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:836120/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:1267813/1‑39 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGt                                  >  1:237891/1‑39 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:568136/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:2504598/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:254919/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:2444103/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:499535/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:533582/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:2167031/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:61354/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:754385/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:854435/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:956347/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:2425783/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:2424828/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1008422/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:2157217/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1843012/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1759005/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1699063/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1682191/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1655058/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1589448/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1548599/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1329921/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1312763/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1269804/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1222847/1‑65 (MQ=255)       aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg  >  1:1017838/1‑65 (MQ=255)                                    |                                    CTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAG  >  W3110S.gb/505144‑505214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑