Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,875,612	G→A	D126N (GAT→AAT)	iap →	aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,875,612	0	G	A	100.0%	99.6 / NA	30	D126N (GAT→AAT)	iap	aminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/22);  total (8/22)

TACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCG  >  W3110S.gb/2875593‑2875672                    |                                                             tACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                 >  1:837793/1‑65 (MQ=255) tACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                 >  1:787675/1‑65 (MQ=255) tACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                 >  1:662972/1‑65 (MQ=255) tACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                 >  1:61034/1‑65 (MQ=255) tACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                 >  1:60884/1‑65 (MQ=255) tACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                 >  1:390552/1‑65 (MQ=255) tACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                 >  1:2254563/1‑65 (MQ=255) tACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATATCGGCGGGCTGACGTTACAAGGa                  >  1:1215775/1‑64 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:2245009/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:973655/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:648721/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:641685/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:633194/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:620827/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:375784/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:2502003/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:106639/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:2097259/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:2047712/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:1989693/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:1922109/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:1873973/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:186467/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:1804598/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:1755024/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:1549256/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:1478807/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:1436497/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:1277500/65‑1 (MQ=255)                gAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg  <  1:112493/65‑1 (MQ=255)                    |                                                             TACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCG  >  W3110S.gb/2875593‑2875672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑