Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,894,340	C→T	W32* (TGG→TGA)	ygcQ ←	predicted flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,894,340	0	C	T	100.0%	30.3 / NA	10	W32* (TGG→TGA)	ygcQ	predicted flavoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/0);  total (10/0)

GCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTT  >  W3110S.gb/2894324‑2894388                 |                                                 gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCTGCCAGCCAGCtt  >  1:1133291/1‑65 (MQ=255) gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCtt  >  1:1032924/1‑65 (MQ=255) gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCtt  >  1:1392769/1‑65 (MQ=255) gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCtt  >  1:1886845/1‑65 (MQ=255) gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCtt  >  1:2089717/1‑65 (MQ=255) gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCtt  >  1:2274709/1‑65 (MQ=255) gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCtt  >  1:2427690/1‑65 (MQ=255) gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCtt  >  1:30712/1‑65 (MQ=255) gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCtt  >  1:597717/1‑65 (MQ=255) gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCt   >  1:1343254/1‑64 (MQ=255)                 |                                                 GCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTT  >  W3110S.gb/2894324‑2894388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑