Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,927,326	G→A	V148M (GTG→ATG)	sdaC →	predicted serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,927,326	0	G	A	100.0%	157.5 / NA	47	V148M (GTG→ATG)	sdaC	predicted serine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/4);  total (43/4)

TCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGA  >  W3110S.gb/2927269‑2927377                                                          |                                                    tctcACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                              <  1:973759/65‑1 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGt                                  >  1:1735415/1‑58 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCtt                            >  1:1056174/1‑64 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCtt                            >  1:646237/1‑64 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCtt                            >  1:1146006/1‑64 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTc                           >  1:898911/1‑65 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTc                           >  1:2372829/1‑65 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTc                           >  1:2476641/1‑65 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTc                           >  1:1976214/1‑65 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTc                           >  1:1030463/1‑65 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTc                           >  1:271314/1‑65 (MQ=255)                    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTc                           >  1:2479417/1‑65 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:982358/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:2316980/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:2332375/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:256219/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:358033/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:794849/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:823605/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:927518/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:934708/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:2203667/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:2147851/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:1871588/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:1739276/1‑63 (MQ=255)                                       tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGt          >  1:1461418/1‑63 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc    >  1:1712528/1‑64 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:1270735/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:1334385/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:1430070/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:145745/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:868156/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:84612/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:1662123/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:499785/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:413608/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:1727828/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:352783/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:281171/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:1748285/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:2525709/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:2062203/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:2423440/1‑65 (MQ=255)                                            cGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg   >  1:2369470/1‑65 (MQ=255)                                             gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa  <  1:1378090/65‑1 (MQ=255)                                             gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa  <  1:964318/65‑1 (MQ=255)                                             gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa  <  1:2314161/65‑1 (MQ=255)                                                          |                                                    TCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGA  >  W3110S.gb/2927269‑2927377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑