Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,960,928	T→C	K53E (AAG→GAG)	recC ←	exonuclease V (RecBCD complex), gamma chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,960,928	0	T	C	100.0%	178.5 / NA	47	K53E (AAG→GAG)	recC	exonuclease V (RecBCD complex), gamma chain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/0);  total (47/0)

AACTTTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATCTCT  >  W3110S.gb/2960924‑2960989     |                                                              aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:456328/1‑65 (MQ=255) aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:2206317/1‑65 (MQ=255) aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:2047487/1‑65 (MQ=255) aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:784394/1‑65 (MQ=255) aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:1600681/1‑65 (MQ=255) aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:1507565/1‑65 (MQ=255) aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:1389791/1‑65 (MQ=255) aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:258546/1‑65 (MQ=255) aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:1313007/1‑65 (MQ=255) aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:341774/1‑65 (MQ=255) aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtct    >  1:1326936/1‑64 (MQ=255) aaCTCTTGCGACAGGGTCATTAGCAGCCACTGTGCCATACCGGt                        >  1:2409914/1‑44 (MQ=38)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATAc                            >  1:863147/1‑39 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:253686/1‑64 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:1067337/1‑64 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:536245/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:666799/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:698034/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:7133/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:764633/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:723594/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:728257/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:238292/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:826441/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:851198/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:899106/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:900407/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:944036/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:982677/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:991131/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:1979104/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:1159276/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:1171775/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:1191853/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:1380465/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:1398117/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:1546036/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:1588573/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:1660389/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:357147/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:2042054/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:2057550/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:2189651/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:2225155/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:2280769/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:2299108/1‑65 (MQ=255)  aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:292119/1‑65 (MQ=255)     |                                                              AACTTTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATCTCT  >  W3110S.gb/2960924‑2960989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑