Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,971,401	A→T	V373E (GTG→GAG)	ygeD ←	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,971,401	0	A	T	100.0%	84.1 / NA	25	V373E (GTG→GAG)	ygeD	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/2);  total (23/2)

GCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCG  >  W3110S.gb/2971368‑2971460                                  |                                                            gcAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGcc                                   >  1:103484/1‑60 (MQ=255) gcAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCg                               >  1:218763/1‑64 (MQ=255)           cACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGccc                    <  1:2143502/65‑1 (MQ=255)            aCCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGccc                    <  1:1290965/64‑1 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTATCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:2302116/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaaca          >  1:2337200/1‑57 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:60780/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1011609/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:599832/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:532998/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:470288/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:2414166/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:2275426/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:2145862/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:2074600/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1715703/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1693867/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1667680/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1662539/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1549676/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1422029/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1416918/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1412780/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1112735/1‑65 (MQ=255)                             tgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcg  >  1:1092422/1‑65 (MQ=255)                                  |                                                            GCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCG  >  W3110S.gb/2971368‑2971460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑