Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,018,630	G→A	D272N (GAC→AAC)	ssnA →	predicted chlorohydrolase/aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,018,630	0	G	A	100.0%	26.6 / NA	9	D272N (GAC→AAC)	ssnA	predicted chlorohydrolase/aminohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/9);  total (0/9)

TTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCAC  >  W3110S.gb/3018573‑3018637                                                          |        ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAc  <  1:1501175/65‑1 (MQ=255) ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAc  <  1:1542930/65‑1 (MQ=255) ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAc  <  1:1585110/65‑1 (MQ=255) ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAc  <  1:1860949/65‑1 (MQ=255) ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAc  <  1:1997625/65‑1 (MQ=255) ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAc  <  1:2117761/65‑1 (MQ=255) ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAc  <  1:2176232/65‑1 (MQ=255) ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAc  <  1:472354/65‑1 (MQ=255) ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAc  <  1:554718/65‑1 (MQ=255)                                                          |        TTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCAC  >  W3110S.gb/3018573‑3018637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑