Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,021,513	T→A	P514P (CCT→CCA)	xdhD →	fused predicted xanthine/hypoxanthine oxidase, molybdopterin‑binding subunit and Fe‑S binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,021,513	0	T	A	100.0%	42.1 / NA	15	P514P (CCT→CCA)	xdhD	fused predicted xanthine/hypoxanthine oxidase, molybdopterin‑binding subunit and Fe‑S binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/0);  total (15/0)

TGGATTTCCGCGCCAACACTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCG  >  W3110S.gb/3021488‑3021552                          |                                        tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGAcc   >  1:1572446/1‑64 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGAcc   >  1:1993293/1‑64 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGAcc   >  1:818155/1‑64 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:1056578/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:1281059/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:13637/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:1982900/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:2306884/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:2455140/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:270528/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:415787/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:605809/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:770116/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:78857/1‑65 (MQ=255) tGGATTTCCGCGCCAACACTGGCCCATACGGCAACCACTCACTCACCGTACCGTGTAACGGACCg  >  1:830222/1‑65 (MQ=255)                          |                                        TGGATTTCCGCGCCAACACTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCG  >  W3110S.gb/3021488‑3021552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑