Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,065,306	A→G	V175A (GTT→GCT)	ygfI ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,065,306	0	A	G	100.0%	62.7 / NA	18	V175A (GTT→GCT)	ygfI	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/0);  total (18/0)

TCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAA  >  W3110S.gb/3065261‑3065366                                              |                                                             tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaagcga                                                           >  1:1944558/1‑49 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaagcg                                                            >  1:427349/1‑48 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:219517/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:988505/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:928191/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:867561/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:720942/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:587113/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:479069/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:430486/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:348348/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:269686/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:2321652/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:1136607/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:158211/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:1151049/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:1580984/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:2136181/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:1614099/1‑65 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                                           >  1:1896452/1‑65 (MQ=255)                                        cgagaagcgagaaaGCCTACTCGCAGCGGTGGTAGTTCACTGATAGTCAGGt                 >  1:820/1‑52 (MQ=255)                                          agaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:188035/65‑1 (MQ=255)                                          agaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:1683284/65‑1 (MQ=255)                                          agaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:548434/65‑1 (MQ=255)                                          agaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:1903517/65‑1 (MQ=255)                                          agaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:650690/65‑1 (MQ=255)                                          agaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:1501299/65‑1 (MQ=255)                                          agaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:1293446/65‑1 (MQ=255)                                          agaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:1196149/65‑1 (MQ=255)                                           gaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:1684366/64‑1 (MQ=255)                                           gaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:2339388/64‑1 (MQ=255)                                           gaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa  <  1:2176049/64‑1 (MQ=255)                                              |                                                             TCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAA  >  W3110S.gb/3065261‑3065366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑