Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,277,106	A→G	T84A (ACT→GCT)	sohA →	predicted regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,277,106	0	A	G	100.0%	89.9 / NA	25	T84A (ACT→GCT)	sohA	predicted regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/0);  total (25/0)

ACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACA  >  W3110S.gb/3277049‑3277121                                                          |                aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:1224240/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:975763/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:868933/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:740923/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:344985/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:26273/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:2169736/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:2142749/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:1985795/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:1712567/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:165475/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:1572893/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:156045/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:1389302/1‑65 (MQ=255) aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc          >  1:1212647/1‑65 (MQ=255)         tGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaaca  >  1:1850103/1‑65 (MQ=255)         tGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaaca  >  1:169409/1‑65 (MQ=255)         tGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaaca  >  1:2048100/1‑65 (MQ=255)         tGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaaca  >  1:1492531/1‑65 (MQ=255)         tGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaaca  >  1:373880/1‑65 (MQ=255)         tGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaaca  >  1:463170/1‑65 (MQ=255)         tGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaaca  >  1:82975/1‑65 (MQ=255)         tGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaaca  >  1:1243981/1‑65 (MQ=255)         tGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaaca  >  1:901879/1‑65 (MQ=255)         tGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaa    >  1:2104660/1‑63 (MQ=255)                                                          |                ACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACA  >  W3110S.gb/3277049‑3277121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑