Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,351,511	A→G	V457A (GTG→GCG)	arcB ←	hybrid sensory histidine kinase in two‑component regulatory system with ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,351,511	0	A	G	100.0%	316.6 / NA	82	V457A (GTG→GCG)	arcB	hybrid sensory histidine kinase in two‑component regulatory system with ArcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/82);  total (0/82)

CGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATG  >  W3110S.gb/3351451‑3351558                                                             |                                                cgaGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTg                                             <  1:2155540/65‑1 (MQ=255) cgaGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTAC‑‑CCATGACTGTCTTTCGCCTGGt                                           <  1:678761/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGTTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1510288/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1402083/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1706217/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:2049189/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:2072610/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1518072/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:2239029/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1486442/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1486417/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1483527/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:291080/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1455034/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1375105/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:382519/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:50367/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:790905/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1180158/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1145045/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:880303/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1066331/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1052804/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:916214/65‑1 (MQ=255)   agaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1003033/65‑1 (MQ=255)    gaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1012317/64‑1 (MQ=255)    gaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:351738/64‑1 (MQ=255)    gaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1727115/64‑1 (MQ=255)    gaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1017351/64‑1 (MQ=255)     aaaCGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTTTCTTTCGCCTGGt                                           <  1:209156/63‑1 (MQ=255)                ccAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt                                           <  1:1304122/52‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:1590420/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:2455661/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:77017/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:2528516/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:81201/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:157618/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:1458299/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:841885/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:745073/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:1994018/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:463028/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:1822634/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:1770577/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:569660/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:1698755/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGTCTATCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:2502024/65‑1 (MQ=255)                            ccggtgGCAGGTTTACCGCCATGACTGACTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAg                  <  1:1269156/65‑1 (MQ=255)                                           ccGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGaa    <  1:567174/64‑1 (MQ=255)                                           ccGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGaa    <  1:1325833/64‑1 (MQ=255)                                           ccGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGaa    <  1:673138/64‑1 (MQ=255)                                           ccGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGaa    <  1:2520825/64‑1 (MQ=255)                                           ccGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGaa    <  1:1516329/64‑1 (MQ=255)                                           ccGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGaa    <  1:223535/64‑1 (MQ=255)                                           ccGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGaa    <  1:824237/64‑1 (MQ=255)                                           ccGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGaa    <  1:2045597/64‑1 (MQ=255)                                           ccGCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGaa    <  1:1995381/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:795371/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:801311/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:1287704/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:860632/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:867481/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:1072264/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:925227/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:971860/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:2163485/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:1725247/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:1654925/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:1818384/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:1832974/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:1934454/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:1587662/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:2107724/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:512406/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:2219718/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:221990/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:2356622/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:2487950/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:1309237/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:391316/64‑1 (MQ=255)                                             gCCATGACTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGATCATCCTGCGGAATg  <  1:1867491/64‑1 (MQ=255)                                                   aCTGTCTTTCGCCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATg  <  1:329223/58‑1 (MQ=255)                                                             |                                                CGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATG  >  W3110S.gb/3351451‑3351558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑