Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	686,526	A→G	I189T (ATC→ACC)	gltJ ←	glutamate and aspartate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	686,526	0	A	G	100.0%	270.1 / NA	72	I189T (ATC→ACC)	gltJ	glutamate and aspartate transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (55/17);  total (55/17)

GCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGC  >  W3110S.gb/686475‑686582                                                    |                                                         gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:103475/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:223714/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:2254205/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:201284/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:2272546/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:1913737/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:40340/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:406259/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:465143/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:1315921/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:897608/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:887440/1‑65 (MQ=255) gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGAt                                             >  1:1206367/1‑65 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCAcc                             >  1:1291164/1‑58 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:1824067/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:2199880/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:2416703/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:2506592/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:1916424/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:1182455/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:1841833/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:1825603/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:829944/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:816657/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:153042/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:1455964/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:632225/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:343132/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:1422692/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:724098/1‑64 (MQ=255)                        cGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc                       >  1:751253/1‑64 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGTAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:2194278/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:667795/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:781804/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:1070959/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:2238459/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:886134/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:275623/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:300893/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:2106421/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:1601866/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:1625452/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:1804815/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:1955565/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:1221127/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:1135354/65‑1 (MQ=255)                                  gccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:2090158/65‑1 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCCCCAGGt                         >  1:1802508/1‑51 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:1141273/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:793232/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:2198130/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:722421/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:9844/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:668245/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:145077/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:654784/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:1453768/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:1577921/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:405077/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:1902890/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:245108/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:1956409/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc                    >  1:2074665/1‑56 (MQ=255)                                   ccATATCCACCAGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGa            <  1:1345679/64‑1 (MQ=255)                                            ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTTACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:440666/1‑65 (MQ=255)                                            ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCa                               >  1:198755/1‑36 (MQ=255)                                            ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:462526/1‑65 (MQ=255)                                            ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:137672/1‑65 (MQ=255)                                            ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:1271836/1‑65 (MQ=255)                                            ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:1122052/1‑65 (MQ=255)                                            ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcgg   >  1:440662/1‑64 (MQ=255)                                            ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcg    >  1:1396658/1‑63 (MQ=255)                                                    |                                                         GCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGC  >  W3110S.gb/686475‑686582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑