BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,374,409	A→G	intergenic (‑65/‑315)	nanR ← / → dcuD	DNA‑binding transcriptional dual regulator/predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,374,409	0	A	G	100.0%	282.5 / NA	74	intergenic (‑65/‑315)	nanR/dcuD	DNA‑binding transcriptional dual regulator/predicted transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/50); total (24/50)

TTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAAATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGAATAAT  >  W3110S.gb/3374363‑3374461

ttAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTctct                                     >  1:761572/1‑64 (MQ=255)

ttAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:1086566/1‑65 (MQ=255)

ttAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:710917/1‑65 (MQ=255)

ttAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:335920/1‑65 (MQ=255)

ttAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:2179014/1‑65 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:164420/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:2116205/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:1698011/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:1817370/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:1880101/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:1935358/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:1004833/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:2148004/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:2184456/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:2295862/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:386698/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:387618/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:457070/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:757608/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:856208/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:1089562/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:1635880/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:1605853/1‑62 (MQ=255)

   aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCtt                                    >  1:1133408/1‑62 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGTAGGGTTTTCTCTTTGTGcc                              <  1:538769/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1193578/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2283927/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1643955/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2297738/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2303126/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2336493/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2444313/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2490827/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2504394/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:314342/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1281210/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:350227/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:397849/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1292083/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:552350/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1157033/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1128862/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:761696/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:811743/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:98777/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2264174/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1690145/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:169350/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1904253/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1568880/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1937148/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2013342/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2022695/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2278322/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:2224826/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1553411/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:155727/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1498092/65‑1 (MQ=255)

      tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGAGcc                              <  1:2198365/65‑1 (MQ=255)

       catcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:1345046/64‑1 (MQ=255)

                         acaGGATTGTAAGTAGGGGAAGATGGCAGTGTTTTCTCTTTGTGcc                              <  1:1245154/46‑1 (MQ=255)

                           aGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                              <  1:374535/44‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:1954245/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:854050/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:1715499/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:1831282/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:609556/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:49088/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:2001292/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:127212/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:212295/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:2034142/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:2060087/64‑1 (MQ=255)

                                   aaGTAGGGGAAGATGGCAGGGGTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGaataat  <  1:162034/64‑1 (MQ=255)

TTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAAATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGAATAAT  >  W3110S.gb/3374363‑3374461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`