BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,404,742	G→A	intergenic (+429/‑549)	yhdH → / → accB	predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,404,742	0	G	A	100.0%	415.4 / NA	122	intergenic (+429/‑549)	yhdH/accB	predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (86/36); total (86/36)

Only 100 of 122 total aligned reads displayed.

CATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGA  >  W3110S.gb/3404690‑3404803

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:2161087/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:1761030/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:1793862/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:1678860/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:1639557/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:1551607/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:417114/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:41445/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:1451940/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:1444065/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:2034506/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:134094/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:1323579/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:2200874/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:377162/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:1255438/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:2432631/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:1071073/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:952335/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:113939/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    <  1:262787/64‑1 (MQ=255)

cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAAACACTGCGTAATgt                                                    <  1:1025184/64‑1 (MQ=255)

        tCTATCCAGACGATTCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATca                                           >  1:1046824/1‑64 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgtcgt                                                 >  1:100530/1‑59 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgtcgt                                                 >  1:1413664/1‑59 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt                                                    >  1:176503/1‑56 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATg                                                     >  1:2328225/1‑55 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:316879/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:2331491/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:439969/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:2006504/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:278218/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:2443006/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:2035960/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:412279/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:2060888/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:380754/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:2121711/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:1010158/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:339729/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:2361514/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:2336170/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:916608/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:795868/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:76496/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:825447/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:1199885/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:600502/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:1164781/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:1433612/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:559365/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:556400/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:494072/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:54458/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:1738993/1‑65 (MQ=255)

        tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt                                           >  1:1745270/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgtcgtc                                                >  1:907046/1‑54 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTggc                                     >  1:2071497/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:1124651/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:109386/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:336627/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:1180658/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:1336971/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:135284/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:397466/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:1421286/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:1469627/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:1513379/1‑65 (MQ=255)

              cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTcgc                                     >  1:472885/1‑65 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATgtcgtc                                                >  1:1286441/1‑39 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATgtcgt                                                 >  1:2248581/1‑38 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCTTCTATCTTGTCGCGATCCTGGCATccc                       >  1:221507/1‑64 (MQ=38)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATGTTGTCGCGATCCTGGCATccc                       >  1:141206/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATcc                                >  1:348379/1‑55 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:721874/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:956236/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:2274687/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:1036013/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:123899/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:1360914/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:175948/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:1968428/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:2399400/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:2050480/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:2253121/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATacc                       >  1:683998/1‑64 (MQ=255)

                             aaaTTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTAGCGATcc                                >  1:1299643/1‑55 (MQ=38)

                             aaaTTGGGTAATCCCCAGCAACCACTCCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATcc                        >  1:1322373/1‑63 (MQ=255)

                             aaaTTGGGTAATCCACAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                       >  1:2056933/1‑64 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa  <  1:1616838/65‑1 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa  <  1:1528066/65‑1 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa  <  1:1394649/65‑1 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa  <  1:2028789/65‑1 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa  <  1:212024/65‑1 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa  <  1:1920543/65‑1 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa  <  1:1181933/65‑1 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa  <  1:1160227/65‑1 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa  <  1:1080175/65‑1 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa  <  1:2009013/65‑1 (MQ=255)

                                                 aCCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGAATCCCTACATTATTTGTGGTCTGTGa  <  1:1700484/65‑1 (MQ=255)

CATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGA  >  W3110S.gb/3404690‑3404803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`