Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,442,070	A→G	T497A (ACG→GCG)	thiC →	thiamin (pyrimidine moiety) biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,442,070	0	A	G	100.0%	91.8 / NA	26	T497A (ACG→GCG)	thiC	thiamin (pyrimidine moiety) biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/9);  total (17/9)

CGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCT  >  W3110S.gb/3442011‑3442122                                                            |                                                     cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg                                                    <  1:1466546/62‑1 (MQ=255) cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg                                                    <  1:1524640/62‑1 (MQ=255) cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg                                                    <  1:1905642/62‑1 (MQ=255) cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg                                                    <  1:1920983/62‑1 (MQ=255) cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg                                                    <  1:2205892/62‑1 (MQ=255) cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg                                                    <  1:219072/62‑1 (MQ=255)          ttGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCAt                                        <  1:1960102/65‑1 (MQ=255)                                      cagcgcGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:1683325/3‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCAt                                        >  1:2055691/1‑37 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGtt             >  1:2272772/1‑64 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:290474/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:688331/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:358231/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:372795/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:428933/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:432463/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:432957/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:1052122/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:281852/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:2166715/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:2123102/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:1911285/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa            >  1:1378003/1‑65 (MQ=255)                                      cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAAAAAAGAAGATGTTa            >  1:523220/1‑65 (MQ=255)                                                ctctGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCt  <  1:2356926/65‑1 (MQ=255)                                                ctctGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCt  <  1:1834989/65‑1 (MQ=255)                                                            |                                                     CGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCT  >  W3110S.gb/3442011‑3442122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑