Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,473,895	A→G	E162G (GAG→GGG)	trmA →	tRNA (uracil‑5‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,473,895	0	A	G	100.0%	332.9 / NA	90	E162G (GAG→GGG)	trmA	tRNA (uracil‑5‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (80/10);  total (80/10)

CATCTGATTGGTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGA  >  W3110S.gb/3473861‑3473948                                   |                                                      cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTc                          >  1:936007/1‑64 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTc                          >  1:2410942/1‑64 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTc                          >  1:1099791/1‑64 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:427897/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1574638/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:745297/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1645220/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1693055/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1711871/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:662494/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:602174/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:504288/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1708999/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1933205/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1949211/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:2447039/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:2231778/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:2400364/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:860723/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1096568/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:885659/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1113331/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:90484/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:890977/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1177537/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1480841/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:142329/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:762606/1‑65 (MQ=255) cATCTGATTGGTCGGGCAACGAAAACCAAAAACGGGCTg                                                   >  1:390041/1‑39 (MQ=37) cATCTGATAGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCt                         >  1:1875105/1‑65 (MQ=255)   tCTGATTGGTCTGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:2048108/1‑43 (MQ=37)   tCTGATTGGTCGGGCAACGAAAACCAAGATCGGGCTGGATCAg                                             >  1:2327741/1‑43 (MQ=38)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:382778/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:2306789/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:739854/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:1668944/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:2450522/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:1560052/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:1373099/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:678419/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:894714/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:433311/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:1734150/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:1329621/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:590315/1‑43 (MQ=255)   tCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAg                                             >  1:1837567/1‑43 (MQ=255)                   aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga       <  1:1031180/65‑1 (MQ=255)                   aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga       <  1:2443522/65‑1 (MQ=255)                   aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga       <  1:338011/65‑1 (MQ=255)                   aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga       <  1:94136/65‑1 (MQ=255)                   aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga       <  1:899160/65‑1 (MQ=255)                   aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga       <  1:2293799/65‑1 (MQ=255)                   aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga       <  1:2113260/65‑1 (MQ=255)                   aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga       <  1:1726862/65‑1 (MQ=255)                   aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga       <  1:1816242/65‑1 (MQ=255)                   aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga       <  1:1925447/65‑1 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGt                           >  1:1845059/1‑39 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:165341/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1044972/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:746217/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1567510/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:858042/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1566078/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:863391/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:880428/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1508388/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:150191/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1328971/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1322175/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1127390/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:906762/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1060207/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:2321518/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1886823/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:2010146/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:2543113/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:294105/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1986735/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:377327/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1930515/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:392485/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1886988/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:735457/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:484347/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1869738/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:577343/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:611329/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:629852/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga  >  1:1709480/1‑64 (MQ=255)                         aCCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatg   >  1:1627522/1‑63 (MQ=255)                                   |                                                      CATCTGATTGGTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGA  >  W3110S.gb/3473861‑3473948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑