Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,497,705	T→G	R20R (CGT→CGG)	gldA →	glycerol dehydrogenase, NAD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,497,705	0	T	G	100.0%	151.1 / NA	40	R20R (CGT→CGG)	gldA	glycerol dehydrogenase, NAD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/33);  total (7/33)

ACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGT  >  W3110S.gb/3497650‑3497742                                                        |                                      aCCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGaa                              >  1:1295456/1‑65 (MQ=255) aCCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGaa                              >  1:2372254/1‑65 (MQ=255) aCCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGaa                              >  1:1842243/1‑65 (MQ=255) aCCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGaa                              >  1:849177/1‑65 (MQ=255) aCCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGaa                              >  1:897942/1‑65 (MQ=255) aCCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGaa                              >  1:1445279/1‑65 (MQ=255) aCCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGaa                              >  1:910135/1‑65 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:823477/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:682160/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:58968/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:547683/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:416427/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:410700/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:311022/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:2526286/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:2455878/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:2446562/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:99329/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:2275175/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:2165841/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:2053293/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:2021303/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:2019550/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:1930713/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:1924336/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:1494696/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:1923990/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:1504207/65‑1 (MQ=255)                cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:1686848/65‑1 (MQ=255)                          aTACATCCAGCGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTg               <  1:2140344/55‑1 (MQ=255)                             cATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt  <  1:1105726/65‑1 (MQ=255)                             cATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt  <  1:218393/65‑1 (MQ=255)                             cATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt  <  1:1441004/65‑1 (MQ=255)                             cATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt  <  1:151209/65‑1 (MQ=255)                             cATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt  <  1:764601/65‑1 (MQ=255)                             cATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt  <  1:638041/65‑1 (MQ=255)                             cATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt  <  1:1813913/65‑1 (MQ=255)                             cATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt  <  1:543755/65‑1 (MQ=255)                             cATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt  <  1:485057/65‑1 (MQ=255)                             cATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt  <  1:1947459/65‑1 (MQ=255)                                                        |                                      ACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGT  >  W3110S.gb/3497650‑3497742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑