Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,500,231	C→T	A115T (GCG→ACG)	yijE ←	predicted permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,500,231	0	C	T	100.0%	89.0 / NA	29	A115T (GCG→ACG)	yijE	predicted permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/29);  total (0/29)

AGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATCACCACCCAGAACGGCATGGTATAGCT  >  W3110S.gb/3500172‑3500267                                                            |                                     aGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGa                                 <  1:2208046/65‑1 (MQ=255) aGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGa                                 <  1:1156666/65‑1 (MQ=255) aGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGa                                 <  1:533748/65‑1 (MQ=255) aGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGa                                 <  1:1896740/65‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:1040038/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:259877/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:2539208/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:2175451/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:435226/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:1986087/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:548001/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:680316/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:839904/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:1587614/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:1498050/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:1320113/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:1311733/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:846580/61‑1 (MQ=255)                         ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:1080490/61‑1 (MQ=255)                           gCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc             <  1:1803550/59‑1 (MQ=255)                               aCGTCGCAGGCGTTCACCGAGAAACAACGTGGCGa                                 <  1:1411788/35‑1 (MQ=255)                                cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt  <  1:1065883/65‑1 (MQ=255)                                cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt  <  1:339708/65‑1 (MQ=255)                                cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt  <  1:2170793/65‑1 (MQ=255)                                cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt  <  1:2152184/65‑1 (MQ=255)                                cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt  <  1:1694468/65‑1 (MQ=255)                                cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt  <  1:1154225/65‑1 (MQ=255)                                   cGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt  <  1:1871361/62‑1 (MQ=255)                                               ccGAGAAACAACGTGGAGAAAATCACCACCCAGAACGGc             <  1:1911704/39‑1 (MQ=37)                                                            |                                     AGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATCACCACCCAGAACGGCATGGTATAGCT  >  W3110S.gb/3500172‑3500267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑