| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,504,714 | G→A | F711F (TTC→TTT) | metL ← | fused aspartokinase II and homoserine dehydrogenase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,504,714 | 0 | G | A | 100.0% | 21.6 / NA | 8 | F711F (TTC→TTT) | metL | fused aspartokinase II and homoserine dehydrogenase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/8); total (0/8) | |||||||||||
GCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGATGCTGCCGCCT > W3110S.gb/3504671‑3504735 | gCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:1619910/65‑1 (MQ=255)gCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:1622847/65‑1 (MQ=255)gCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:2040667/65‑1 (MQ=255)gCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:204311/65‑1 (MQ=255)gCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:2141352/65‑1 (MQ=255)gCCGGTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:2043867/65‑1 (MQ=255) ccATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAACTGGGCGATGCTGCCGCCt < 1:664706/56‑1 (MQ=255) cGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:2446430/35‑1 (MQ=255) | GCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGATGCTGCCGCCT > W3110S.gb/3504671‑3504735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |