Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,572,896 C→T R171* (CGA→TGA)  ompL → predicted outer membrane porin L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,572,8960CT100.0% 361.6 / NA 105R171* (CGA→TGA) ompLpredicted outer membrane porin L
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/71);  total (34/71)

Only 100 of 105 total aligned reads displayed.

ATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAT  >  W3110S.gb/3572843‑3572945
                                                     |                                                 
aTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGa                                         <  1:901450/64‑1 (MQ=255)
aTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGa                                         <  1:1076798/64‑1 (MQ=255)
aTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGa                                         <  1:1093737/64‑1 (MQ=255)
aTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGa                                         <  1:824973/64‑1 (MQ=255)
aTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGa                                         <  1:2422447/64‑1 (MQ=255)
aTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGa                                         <  1:693181/64‑1 (MQ=255)
aTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGa                                         <  1:1717927/64‑1 (MQ=255)
aTTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGa                                         <  1:1218141/64‑1 (MQ=255)
  tttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACtt                                      <  1:2293645/65‑1 (MQ=255)
        aTCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAtag                                <  1:1025163/65‑1 (MQ=255)
        aTCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAtag                                <  1:1518491/65‑1 (MQ=255)
        aTCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAtag                                <  1:2541274/65‑1 (MQ=255)
        aTCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAtag                                <  1:1238918/65‑1 (MQ=255)
        aTCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAtag                                <  1:1217093/65‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:646499/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:579706/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:503726/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:2049239/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:1354006/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:486564/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:1139355/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:418781/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:2457302/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:310464/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:1494229/64‑1 (MQ=255)
                         cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:119967/64‑1 (MQ=255)
                          tataCATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:1883237/63‑1 (MQ=255)
                          tataCATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:2177764/63‑1 (MQ=255)
                          tataCATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:7377/63‑1 (MQ=255)
                          tataCATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:1464393/63‑1 (MQ=255)
                           ataCATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:2370320/62‑1 (MQ=255)
                            taCATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                <  1:2068196/61‑1 (MQ=255)
                                      ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt  <  1:219107/65‑1 (MQ=255)
                                      ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt  <  1:2035784/65‑1 (MQ=255)
                                      ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt  <  1:2460735/65‑1 (MQ=255)
                                      ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt  <  1:333177/65‑1 (MQ=255)
                                      ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt  <  1:466257/65‑1 (MQ=255)
                                      ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt  <  1:489306/65‑1 (MQ=255)
                                      ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt  <  1:683468/65‑1 (MQ=255)
                                      ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt  <  1:874192/65‑1 (MQ=255)
                                      ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt  <  1:1332085/65‑1 (MQ=255)
                                      acacaTTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt  <  1:865417/64‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:676700/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1355174/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:473262/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1365687/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:428664/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:628633/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1490765/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:310104/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1505884/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1576588/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:2417632/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1251954/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1647067/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:2274512/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:2258815/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:112356/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1960119/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1990414/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1841146/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1135683/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:808890/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:747371/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:2217701/40‑1 (MQ=255)
                                             aCTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:2271701/40‑1 (MQ=255)
                                              cTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:1599218/39‑1 (MQ=255)
                                              cTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGaa                    <  1:722607/39‑1 (MQ=255)
                                                 cATGTGATTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGa     >  1:1481474/1‑51 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:632735/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:635718/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:1260436/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:1296495/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:831983/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:345961/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:721133/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:533063/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:104019/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:134809/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:1883720/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                >  1:1704506/1‑40 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:1913655/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:516575/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:1936408/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:1879731/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:2178898/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:800253/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:763851/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:1672709/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:1150906/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:2233886/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:2258776/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:116837/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:1642393/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:2372331/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:1616387/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:2528359/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:1898570/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa    >  1:1447298/1‑52 (MQ=255)
                                                 cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATAGGGa     >  1:2453705/1‑51 (MQ=255)
                                                     |                                                 
ATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAT  >  W3110S.gb/3572843‑3572945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑