Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	710,341	C→T	P44S (CCA→TCA)	ybfN →	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	710,341	0	C	T	100.0%	14.4 / NA	6	P44S (CCA→TCA)	ybfN	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/5);  total (1/5)

AGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAG  >  W3110S.gb/710301‑710391                                         |                                                   agagGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCaga                            >  1:2299374/1‑65 (MQ=255)                           ccGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag  <  1:1709239/65‑1 (MQ=255)                           ccGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag  <  1:363392/65‑1 (MQ=255)                           ccGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag  <  1:742771/65‑1 (MQ=255)                           ccGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag  <  1:752836/65‑1 (MQ=255)                           ccGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag  <  1:821422/65‑1 (MQ=255)                                         |                                                   AGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAG  >  W3110S.gb/710301‑710391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑