Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,587,844	T→C	P624P (CCA→CCG)	polA ←	fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,587,844	0	T	C	100.0%	105.8 / NA	29	P624P (CCA→CCG)	polA	fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/20);  total (9/20)

AGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACG  >  W3110S.gb/3587785‑3587896                                                            |                                                     aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:2145748/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:966097/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:905266/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:804034/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:791634/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:599405/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:436487/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:36448/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:2171873/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:2097881/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:185862/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:1419247/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:1491603/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:1660066/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:1513403/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:1655040/65‑1 (MQ=255) aGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:1618018/65‑1 (MQ=255)  gCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:1123387/64‑1 (MQ=255)  gCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:1649800/64‑1 (MQ=255)             tAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAg                                     >  1:475447/1‑65 (MQ=255)             tAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAg                                     >  1:1578942/1‑65 (MQ=255)             tAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAg                                     >  1:639129/1‑65 (MQ=255)             tAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAg                                     >  1:1760632/1‑65 (MQ=255)             tAGGTCGATTTCAGCTTCGCCAGACCACGATACTCAAGAATCACTTTCGGCAACGGATAGTCCAg                                     >  1:2444715/1‑65 (MQ=255)                     tttCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCaa                                                 <  1:2485551/45‑1 (MQ=255)                                                cAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTgcc   >  1:1985174/1‑62 (MQ=255)                                                cAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACg  >  1:1781864/1‑65 (MQ=255)                                                cAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACg  >  1:768537/1‑65 (MQ=255)                                                cAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACg  >  1:932283/1‑65 (MQ=255)                                                            |                                                     AGCTTGTCGGTGTAGGTCGATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACGTTGACG  >  W3110S.gb/3587785‑3587896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑