Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,614,917	G→A	G513G (GGC→GGT)	ubiB ←	2‑octaprenylphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,614,917	0	G	A	100.0%	22.9 / NA	9	G513G (GGC→GGT)	ubiB	2‑octaprenylphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/0);  total (9/0)

TCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCG  >  W3110S.gb/3614896‑3614960                      |                                            tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg  >  1:105119/1‑65 (MQ=255) tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg  >  1:1299764/1‑65 (MQ=255) tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg  >  1:1339550/1‑65 (MQ=255) tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg  >  1:192505/1‑65 (MQ=255) tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg  >  1:215982/1‑65 (MQ=255) tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg  >  1:569634/1‑65 (MQ=255) tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg  >  1:648150/1‑65 (MQ=255) tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg  >  1:887631/1‑65 (MQ=255) tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg  >  1:890552/1‑65 (MQ=255)                      |                                            TCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCG  >  W3110S.gb/3614896‑3614960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑