Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,714,423	C→T	H61Y (CAC→TAC)	atpI →	ATP synthase, membrane‑bound accesory subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,714,423	0	C	T	100.0%	92.0 / NA	27	H61Y (CAC→TAC)	atpI	ATP synthase, membrane‑bound accesory subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/0);  total (27/0)

CCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGT  >  W3110S.gb/3714390‑3714454                                  |                                ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCTCATACACCAGCGAAAGGCCGGGt  >  1:936913/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:2183811/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:963508/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:929477/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:890099/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:820650/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:772088/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:686759/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:49456/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:2513264/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:249794/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:2384329/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:2377784/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:1462181/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:2165102/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:2102296/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:2064297/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:196408/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:1887484/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:1885286/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:186707/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:1798709/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:1784171/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:1762259/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:1555230/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt  >  1:1547002/1‑65 (MQ=255) ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACAAGCGAAAGGCCGGGt  >  1:621768/1‑65 (MQ=255)                                  |                                CCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGT  >  W3110S.gb/3714390‑3714454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑