Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,722,454	T→A	C324* (TGT→TGA)	glmU →	fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,722,454	0	T	A	100.0%	101.1 / NA	32	C324* (TGT→TGA)	glmU	fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (32/0);  total (32/0)

ATGATTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGTACCATT  >  W3110S.gb/3722396‑3722460                                                           |       atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAGCCAtt  >  1:739621/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:2295887/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:994792/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:939908/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:903741/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:803820/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:720544/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:638010/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:336499/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:276055/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:2511071/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:2508173/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:2504104/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:2367367/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:2346083/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1010710/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:2113127/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:2109982/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:2027695/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1844361/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1740391/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1689479/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:165984/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1654324/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1511009/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1500545/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1406549/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1087624/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1078420/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:1069313/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAtt  >  1:106097/1‑65 (MQ=255) atgatTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGAACCAt   >  1:272166/1‑64 (MQ=255)                                                           |       ATGATTGCGAAATCAGTCCGTATACCGTTGTGGAAGATGCGAATCTGGCAGCGGCCTGTACCATT  >  W3110S.gb/3722396‑3722460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑