Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,767,300	A→G	I166V (ATA→GTA)	dgoA →	2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,767,300	0	A	G	100.0%	12.5 / NA	5	I166V (ATA→GTA)	dgoA	2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/3);  total (2/3)

TCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCG  >  W3110S.gb/3767243‑3767351                                                          |                                                    tCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGc                                              >  1:1186382/1‑65 (MQ=255) tCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGc                                              >  1:1569484/1‑65 (MQ=255)                                             ccTGGCGCAGTGGGTAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCg  <  1:1324183/65‑1 (MQ=255)                                             ccTGGCGCAGTGGGTAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCg  <  1:548131/65‑1 (MQ=255)                                             ccTGGCGCAGTGGGGAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCg  <  1:1051377/65‑1 (MQ=255)                                                          |                                                    TCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCG  >  W3110S.gb/3767243‑3767351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑