| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,777,889 | T→C | V359A (GTG→GCG) | glvC → | arbutin specific enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,777,889 | 0 | T | C | 94.4% | 57.7 / ‑5.6 | 18 | V359A (GTG→GCG) | glvC | arbutin specific enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (0/17); minor base G (0/1); total (0/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAG > W3110S.gb/3777842‑3777906 | cttGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:1953146/63‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGGGGGCAACATGGGCGGAg < 1:1900227/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:1927813/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:915796/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:702803/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:433719/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:334046/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:2430392/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:2168131/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:2028668/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:1252843/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:1862369/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:1566566/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:1300455/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:1295254/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:1283508/65‑1 (MQ=255)cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:1281883/65‑1 (MQ=255) gTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg < 1:1762756/64‑1 (MQ=255) | CGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAG > W3110S.gb/3777842‑3777906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |