Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	847,104	A→G	R146R (CGT→CGC)	glnP ←	glutamine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	847,104	0	A	G	100.0%	96.6 / NA	27	R146R (CGT→CGC)	glnP	glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/0);  total (27/0)

TGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGA  >  W3110S.gb/847073‑847137                                |                                  tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGcc                       >  1:675504/1‑44 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGc                        >  1:1911683/1‑43 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:1947016/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:818638/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:763455/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:446154/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:440687/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:412470/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:258798/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:2488076/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:2410071/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:2317611/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:2110282/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:2034995/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:1977224/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:1874427/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:178337/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:1768386/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:1678504/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:1597207/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:1566437/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:1519540/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:147059/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:1455760/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:1439506/1‑65 (MQ=255) tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa  >  1:130290/1‑65 (MQ=255) tgatCCACTGGTTACACAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAg     >  1:1252090/1‑62 (MQ=255)                                |                                  TGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGA  >  W3110S.gb/847073‑847137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑