Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,103,846 T→C Y5H (TAC→CAC)  ompR → DNA‑binding response regulator in two‑component regulatory system with EnvZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,103,8460TC100.0% 535.3 / NA 141Y5H (TAC→CAC) ompRDNA‑binding response regulator in two‑component regulatory system with EnvZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (82/59);  total (82/59)

Only 100 of 141 total aligned reads displayed.

CTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTAT  >  W3110S.gb/4103798‑4103902
                                                |                                                        
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGCGAACCACAAGATTCtggtgg                                           >  1:1769583/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:765238/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:1729887/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:171063/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:1786194/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:1801803/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:1631715/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:1981573/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:2014838/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:204647/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:2113863/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:1285913/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:2126830/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:260071/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:272314/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                           >  1:344294/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATACtggtgg                                           >  1:348707/1‑64 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:2264368/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:2061309/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:472110/64‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:1763458/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:1012025/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:597618/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:569085/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:564279/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:1894935/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:2027589/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:2030683/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:2058217/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:2526209/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:2421072/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:1006388/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:105323/40‑1 (MQ=255)
                              caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:151825/40‑1 (MQ=255)
                               aaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:982369/39‑1 (MQ=255)
                                aacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:2492986/38‑1 (MQ=255)
                                aacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                     <  1:1149478/38‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGctgctgta         <  1:174139/65‑3 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:1755091/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:120847/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:311646/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:84173/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:31530/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:770109/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:1304126/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:1418513/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:1424081/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:693265/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:1688607/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:102838/65‑1 (MQ=255)
                                 acaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:1954315/65‑1 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGCCGACATGCGCCTGCGTGCGCTGCTGGa         >  1:1280314/1‑64 (MQ=39)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTgcgc                >  1:182974/1‑57 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:378520/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:358114/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:354088/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:1108119/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:568534/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:590608/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:1015658/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:60273/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:1136444/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:426821/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:813663/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:646352/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:2525781/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:850380/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:1967714/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:1628781/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:1665886/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:1877945/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:2003999/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:1877412/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:1313876/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:1290672/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:2129296/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:2189170/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:2228565/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:888813/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:2306529/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:2320036/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:243790/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:2466264/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa        >  1:2496841/1‑65 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:1195158/64‑1 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:504745/64‑1 (MQ=255)
                                  caaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGa         <  1:1084168/64‑1 (MQ=255)
                                  caaTGAAAGAGAACCACAAGATTCTGGTGGTCGATgacga                                 >  1:1262744/1‑40 (MQ=38)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc       >  1:866641/1‑64 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc       >  1:985951/1‑64 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc       >  1:444015/1‑64 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc       >  1:1117974/1‑64 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc       >  1:2529608/1‑64 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc       >  1:226632/1‑64 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc       >  1:1529744/1‑64 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTCCGTGCGCTGCTgg          >  1:1694097/1‑61 (MQ=255)
                                         agagAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg      >  1:1874558/1‑60 (MQ=255)
                                         agagAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTAt  >  1:384206/1‑64 (MQ=255)
                                         agagAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTAt  >  1:2161080/1‑64 (MQ=255)
                                         agagAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTAt  >  1:1750861/1‑64 (MQ=255)
                                                |                                                        
CTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTAT  >  W3110S.gb/4103798‑4103902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑