Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,256,281	C→A	P62T (CCC→ACC)	ubiC →	chorismate pyruvate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,256,281	0	C	A	100.0%	37.8 / NA	12	P62T (CCC→ACC)	ubiC	chorismate pyruvate lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/12);  total (0/12)

ATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTC  >  W3110S.gb/4256248‑4256312                                  |                                atCCGCGAAGGGTTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:1022588/65‑1 (MQ=255) atCCGCGAAGGGTTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:1380934/65‑1 (MQ=255) atCCGCGAAGGGTTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:1797271/65‑1 (MQ=255) atCCGCGAAGGGTTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:1812900/65‑1 (MQ=255) atCCGCGAAGGGTTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:2038365/65‑1 (MQ=255) atCCGCGAAGGGTTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:2041775/65‑1 (MQ=255) atCCGCGAAGGGTTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:2268802/65‑1 (MQ=255) atCCGCGAAGGGTTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:293720/65‑1 (MQ=255) atCCGCGAAGGGTTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:481644/65‑1 (MQ=255) atCCGCGAAGGGTTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:578620/65‑1 (MQ=255) atCCGCGAAGGGTTTGGCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:2272494/65‑1 (MQ=255) atCCGCGAAGGGGTTGTCGAGCAGAATGAAATCACCGAAGAACTGCCGCTGCTGCCGAAAGAGtc  <  1:1548875/65‑1 (MQ=255)                                  |                                ATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTC  >  W3110S.gb/4256248‑4256312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑