Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	921,181	C→T	T138I (ACA→ATA)	macB →	fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	921,181	0	C	T	100.0%	118.6 / NA	36	T138I (ACA→ATA)	macB	fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/35);  total (1/35)

AGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCG  >  W3110S.gb/921148‑921236                                  |                                                        aGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGggtggt                          <  1:864389/65‑1 (MQ=255) aGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGggtggt                          <  1:2534760/65‑1 (MQ=255) aGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGggtggt                          <  1:571184/65‑1 (MQ=255) aGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGggtggt                          <  1:176481/65‑1 (MQ=255)         cAACGGCTGGGGCTGGAAGCCCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACa                  >  1:426681/1‑65 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACGGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:698504/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATctcg  <  1:324247/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1126794/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:2309479/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:2373447/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:2410325/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:343596/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:662364/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:781100/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:805968/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:862721/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:970906/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:2178443/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:130118/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1317816/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1456022/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1496764/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1556078/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1646567/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1674845/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1694457/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1828282/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1837855/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1961697/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:2141732/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:2237275/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcccg  <  1:324745/64‑1 (MQ=255)                          aGACCGTATAGAGTATTATCCGGCACAGCGTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:961930/64‑1 (MQ=255)                           gACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:129312/63‑1 (MQ=255)                           gACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg  <  1:1205294/63‑1 (MQ=255)                           gACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGGCAGCAACAGCGCGTCAGCATcgcg  <  1:409577/63‑1 (MQ=255)                                  |                                                        AGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCG  >  W3110S.gb/921148‑921236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑