Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,558,086	G→A	A168A (GCG→GCA)	uxuB →	D‑mannonate oxidoreductase, NAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,558,086	0	G	A	100.0%	267.4 / NA	78	A168A (GCG→GCA)	uxuB	D‑mannonate oxidoreductase, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (51/27);  total (51/27)

CACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATA  >  W3110S.gb/4558030‑4558141                                                         |                                                        cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:51449/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:1590301/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:559328/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:648724/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:2209785/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:2236566/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:1771469/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:2119067/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:356287/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:2353147/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:210368/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:1289144/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:253899/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:387361/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:1267052/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:1967817/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:832428/1‑65 (MQ=255) cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCATtgcgtct                                                 >  1:1016284/1‑65 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGATAAAGGGTTGAAAGCGTTTACGGt                <  1:1340125/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:2085089/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:2122041/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:185868/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:376224/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:2138556/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:2241446/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:225742/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:2300219/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:336948/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:252603/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:26448/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:288148/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:293017/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:1270746/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:75684/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:1812458/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:541663/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:1600659/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:1547207/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:138917/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:1484120/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:650171/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:750759/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:1448677/64‑1 (MQ=255)                                   cgATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:750775/64‑1 (MQ=255)                                    gATTGGTTACATCGGCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt                <  1:768108/63‑1 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGtt                               >  1:2194698/1‑36 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGa    >  1:2305341/1‑63 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGAt   >  1:2504627/1‑64 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:90765/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:775960/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:383208/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:317894/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:763773/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:757211/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:927419/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:38335/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:146894/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1075625/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1086020/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1096749/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1174116/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1281987/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1317540/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1356473/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1391817/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1458216/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:302563/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1546568/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1648479/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1649653/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1834902/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1862704/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1995823/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:2023251/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:2130448/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:2314594/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:2409354/1‑65 (MQ=255)                                                cgtcgAAGCATTGCGTCTGCGGCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa  >  1:1375806/1‑65 (MQ=255)                                                         |                                                        CACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATA  >  W3110S.gb/4558030‑4558141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑