Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,630,397	C→A	A274D (GCC→GAC)	serB →	3‑phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,630,397	0	C	A	100.0%	63.6 / NA	19	A274D (GCC→GAC)	serB	3‑phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/19);  total (0/19)

CCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGG  >  W3110S.gb/4630363‑4630427                                   |                               ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:1659324/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:967891/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:801653/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:691176/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:630865/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:2491213/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:2394289/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:2381863/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:2075897/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:1717884/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:13948/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:1358063/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:1296128/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:1266844/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:1263706/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:1170282/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:1096261/65‑1 (MQ=255) ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:1074/65‑1 (MQ=255)  ctcTGGCGCAGACCGTGGCGATTGGCGATGGATACAATGACCTGCCGATGATCAAAGCGGCAgg  <  1:1031724/62‑1 (MQ=255)                                   |                               CCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGG  >  W3110S.gb/4630363‑4630427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑