Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 940,557 A→G P236P (CCA→CCG)  serS → seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb940,5570AG100.0% 331.2 / NA 88P236P (CCA→CCG) serSseryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (61/27);  total (61/27)

TCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTG  >  W3110S.gb/940512‑940575
                                             |                  
tCCGCTGGAAGAAGAAGCAGCCACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1696446/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGCTCCCGACGGCAGAAGTTCCGCTg  >  1:626140/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGaa           >  1:477418/1‑55 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGa            >  1:1038729/1‑54 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGcag  >  1:1811474/1‑62 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCt   >  1:854175/1‑63 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:344349/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:448642/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:432944/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:423034/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:415623/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:394777/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:37987/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2223769/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:297101/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:256296/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2482317/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:247353/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2376695/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2314823/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2308613/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1037747/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:731586/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:981550/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:945339/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:852892/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:837511/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:837365/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:809256/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:770450/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:749416/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:458268/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:71793/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:670765/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:58342/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:564244/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:501162/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:474739/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:459395/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1997095/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1716457/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1616370/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1759836/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1221445/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1794432/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1825849/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1853859/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1879556/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:191689/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1615422/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:194910/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1355416/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2003753/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2011131/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2059841/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2075460/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2086761/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:2089633/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1038025/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  >  1:1475668/1‑64 (MQ=255)
tCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCAAGACGGCAGAAGTTCCGCTg  >  1:1130837/1‑64 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:827766/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:1363367/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:138691/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:723800/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:80618/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:1719985/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:1192565/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:1191682/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:1182294/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:1026405/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:991657/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:142397/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:1533348/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:1545958/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:50462/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:2202127/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:453816/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:381742/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:356606/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:2445519/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:2418025/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:2378793/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:2092004/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:2265671/63‑1 (MQ=255)
 ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:2138565/63‑1 (MQ=255)
           aagaagCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:1492447/53‑1 (MQ=255)
                          gTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg  <  1:736067/38‑1 (MQ=255)
                                             |                  
TCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTG  >  W3110S.gb/940512‑940575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑