| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 970,899 | A→G | P363P (CCA→CCG) | ycaQ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 970,899 | 0 | A | G | 86.0% | 131.4 / 13.4 | 50 | P363P (CCA→CCG) | ycaQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base G (43/0); total (50/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
GCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGC > W3110S.gb/970844‑970954 | gCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACg > 1:556380/1‑65 (MQ=255)gCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACg > 1:2432520/1‑65 (MQ=255)gCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACg > 1:2173747/1‑65 (MQ=255) ccAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACcaacgccaacgc > 1:2302936/1‑65 (MQ=255) ccAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGc > 1:2223757/1‑65 (MQ=255) ccAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGc > 1:2047966/1‑65 (MQ=255) ccAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGc > 1:1822563/1‑65 (MQ=255) ccAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAAAGACAACGc > 1:1262715/1‑65 (MQ=255) cagtAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:324943/4‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGAt > 1:1683681/2‑52 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTgg > 1:1390711/2‑64 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:555571/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:995744/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:2472626/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:249909/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:2501968/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:2506405/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:349685/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:375707/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:478255/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:919327/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:2250034/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:632266/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:665564/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:714430/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:790245/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:860870/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:867836/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:872425/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:882495/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1834482/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1084485/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1263001/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1267563/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1276465/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1530198/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1531726/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1547200/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1752629/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1812582/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1818099/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:2370859/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1924342/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1925913/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1927302/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1931494/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1945419/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1999734/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:1005402/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:2251279/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc > 1:2264735/2‑65 (MQ=255) | GCCAGACAGGCATCCTTGAAGTTATCTCTCTGTGGTTACAGGAAGGTATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGC > W3110S.gb/970844‑970954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |