Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	970,921	C→T	L371L (CTA→TTA)	ycaQ →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	970,921	0	C	T	100.0%	151.1 / NA	43	L371L (CTA→TTA)	ycaQ	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/0);  total (43/0)

TATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGC  >  W3110S.gb/970890‑970954                                |                                  cagtAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:324943/4‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGAt               >  1:1683681/2‑52 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTgg   >  1:1390711/2‑64 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:665564/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:2370859/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:2472626/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:249909/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:2501968/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:2506405/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:349685/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:375707/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:478255/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:555571/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:632266/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:2251279/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:714430/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:790245/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:860870/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:867836/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:872425/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:882495/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:919327/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:995744/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1818099/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1084485/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1263001/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1267563/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1276465/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1530198/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1531726/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1547200/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1752629/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1812582/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:2264735/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1834482/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1924342/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1925913/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1927302/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1931494/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1945419/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1999734/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:2250034/2‑65 (MQ=255) caTTAAACCGACGACAACGCTGCAAAAAGGGTTACGTCAGGCGATTACTGATTTCGCTAACTGgc  >  1:1005402/2‑65 (MQ=255)                                |                                  TATTAAACCAACGACAACGCTGCAAAAAGGGCTACGTCAGGCGATTACTGATTTCGCTAACTGGC  >  W3110S.gb/970890‑970954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑