Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	121,347	T→C	M69V (ATG→GTG)	aroP ←	aromatic amino acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	121,347	0	T	C	100.0%	170.3 / NA	45	M69V (ATG→GTG)	aroP	aromatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/0);  total (45/0)

TAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCC  >  W3110S.gb/121301‑121402                                               |                                                        taAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                       >  1:907132/1‑65 (MQ=255) taAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                       >  1:2263114/1‑65 (MQ=255) taAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                       >  1:902362/1‑65 (MQ=255) taAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                       >  1:1043643/1‑65 (MQ=255) taAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                       >  1:1887960/1‑65 (MQ=255) taAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                       >  1:17380/1‑65 (MQ=255) taAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                       >  1:692508/1‑65 (MQ=255) taAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                       >  1:148165/1‑65 (MQ=255) taAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                       >  1:1482659/1‑65 (MQ=255) taAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                       >  1:727448/1‑65 (MQ=255)                                     tcGACCACCACTTCATCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:773279/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:31542/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:453892/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:329189/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:249524/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:294077/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:2198582/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:602458/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:635592/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:740739/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:751129/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:79638/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:838194/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:904773/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:959601/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1435478/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1083141/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1088702/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1117798/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:121513/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1373052/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1403645/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1420735/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:2394316/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1492794/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1635026/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1765927/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1862575/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:1998541/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:2080784/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:2362734/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGAGAAAACCAGCAATGGCGTaa     >  1:174188/1‑63 (MQ=255)                                     tcGACCACCACTTCACCCAGCTCACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTaa     >  1:82575/1‑63 (MQ=255)                                      cGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAAccc  >  1:2114903/1‑65 (MQ=255)                                      cGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGAGATAAAACCAGCAATGGCGTAAccc  >  1:1309742/1‑65 (MQ=255)                                               |                                                        TAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCC  >  W3110S.gb/121301‑121402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑