Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,093,686	T→C	V130A (GTA→GCA)	ycdT →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,093,686	0	T	C	100.0%	54.4 / NA	16	V130A (GTA→GCA)	ycdT	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/13);  total (3/13)

TCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATG  >  W3110S.gb/1093638‑1093744                                                 |                                                           tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                            >  1:1264829/1‑65 (MQ=255)                           ttGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt                  >  1:2252643/1‑65 (MQ=255)                           ttGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt                  >  1:788596/1‑65 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:1139444/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:1303600/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:1440050/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:1617974/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:1631005/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:1792282/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:1961665/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:2061702/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:2121985/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:2225471/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:462171/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:591464/64‑1 (MQ=255)                                            tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg  <  1:801212/64‑1 (MQ=255)                                                 |                                                           TCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATG  >  W3110S.gb/1093638‑1093744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑