Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,135,828	T→C	D150D (GAT→GAC)	flgF →	flagellar component of cell‑proximal portion of basal‑body rod

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,135,828	0	T	C	100.0%	76.9 / NA	22	D150D (GAT→GAC)	flgF	flagellar component of cell‑proximal portion of basal‑body rod
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/22);  total (0/22)

GATGGCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGT  >  W3110S.gb/1135796‑1135858                                 |                               gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:2093541/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:836559/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:747177/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:377312/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:339673/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:314732/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:2412799/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:231859/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:2174244/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:2120009/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:10178/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:1988175/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:1975234/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:1944309/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:1751814/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:1686111/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:1648289/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:159542/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:1509034/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:1486154/63‑1 (MQ=255) gATGGCACAATCTCGGCGCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:1245764/63‑1 (MQ=255) gATGGCACAATCTCGGCCCTCAATCCGGGCGACCCGGCAAATACGGTTGCGCCAGTAGGGCGt  <  1:2202525/63‑1 (MQ=255)                                 |                               GATGGCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGT  >  W3110S.gb/1135796‑1135858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑