Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,150,273	A→G	intergenic (+5/‑63)	plsX → / → fabH	fatty acid/phospholipid synthesis protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,150,273	0	A	G	100.0%	59.8 / NA	17	intergenic (+5/‑63)	plsX/fabH	fatty acid/phospholipid synthesis protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/17);  total (0/17)

GCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTT  >  W3110S.gb/1150232‑1150295                                          |                       gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:1889840/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:94694/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:931554/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:568224/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:499947/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:332731/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:321516/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:2065711/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:1054474/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:1834796/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:1833585/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:1745697/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:1699527/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:1512206/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:1419124/64‑1 (MQ=255) gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGtt  <  1:1220250/64‑1 (MQ=255)                             ctGCGGTAGCTGGGCGCTGCCAGCGAACTCGCAGtt  <  1:1927104/36‑1 (MQ=37)                                          |                       GCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTT  >  W3110S.gb/1150232‑1150295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑