Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,197,638	G→A	L313L (CTG→CTA)	icd →	isocitrate dehydrogenase, specific for NADP+

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,197,638	0	G	A	100.0%	48.8 / NA	15	L313L (CTG→CTA)	icd	isocitrate dehydrogenase, specific for NADP+
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/15);  total (0/15)

ATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGG  >  W3110S.gb/1197589‑1197652                                                  |               atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:1267334/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:1707865/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:2031163/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:2072135/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:2129577/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:2371461/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:24177/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:298491/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:366299/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:625440/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:731811/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:892593/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:975677/64‑1 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:987291/64‑1 (MQ=255)                    ccTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTgg  <  1:1565870/45‑1 (MQ=255)                                                  |               ATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGG  >  W3110S.gb/1197589‑1197652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑