Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 155,463 A→T *247K (TAA→AAA)  ecpD ← predicted periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb155,4630AT100.0% 352.2 / NA 101*247K (TAA→AAA) ecpDpredicted periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (36/65);  total (36/65)

Only 100 of 101 total aligned reads displayed.

TTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCA  >  W3110S.gb/155415‑155500
                                                |                                     
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:1054171/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:2074078/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:2229839/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:2290392/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:2357923/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:2520638/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:1769511/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:44915/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:478598/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:553872/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:1525741/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:1525236/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:1395414/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:768596/56‑1 (MQ=255)
ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg                                <  1:1293376/56‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTTCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1685224/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:2263651/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:883053/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:909142/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:2422386/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:926874/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:2296572/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:928277/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:2280822/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:351077/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1046195/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:2224692/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:2195089/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:945042/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:2122902/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:2108440/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:313523/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:743092/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:390096/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:441900/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:843823/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:455582/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:475040/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:825977/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:784171/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:5998/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:662042/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:684356/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:750866/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:69878/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:2085197/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:106171/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1208865/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1241566/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1287800/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1309511/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1398241/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1435235/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1470310/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1552454/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1648437/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1678678/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1727660/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1733824/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1793845/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1871007/64‑1 (MQ=255)
                    tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:2051939/64‑1 (MQ=255)
                     ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:1530890/63‑1 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCGTTACCTTCAATTGCGCCACCAAAGTCa  >  1:1306933/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTTCCTTCAATTGCGcc            >  1:39903/1‑54 (MQ=39)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACTTTCAATTGCGCCACCAAAGTc   >  1:723608/1‑63 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt                 >  1:1081531/1‑49 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc   >  1:1818155/1‑63 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc   >  1:2103111/1‑63 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc   >  1:911048/1‑63 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc   >  1:2223981/1‑63 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc   >  1:79669/1‑63 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc   >  1:2232590/1‑63 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc   >  1:2247708/1‑63 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc   >  1:264665/1‑63 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc   >  1:39702/1‑63 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:1910468/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:2369282/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:2474717/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:1206976/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:911059/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:2164002/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:902519/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:1239396/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:2056971/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:282842/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:2327406/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:1879357/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:1563640/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:1315712/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:1317889/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:396476/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:133287/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:2027184/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:622895/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:1665263/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:1942457/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa  >  1:153165/1‑64 (MQ=255)
                      cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACATTCAATTGAGCCACCAAAGTc   >  1:2013671/1‑63 (MQ=255)
                                    ccTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt    <  1:638284/48‑1 (MQ=255)
                                                |                                     
TTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCA  >  W3110S.gb/155415‑155500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑