Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,207,066	A→G	E440E (GAA→GAG)	ymfN →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,207,066	0	A	G	100.0%	182.4 / NA	49	E440E (GAA→GAG)	ymfN	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/32);  total (17/32)

TGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGG  >  W3110S.gb/1207021‑1207120                                              |                                                       tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:164929/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:237707/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:2201061/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:2169413/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:861095/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:2441354/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:2019578/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1999862/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:814639/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:2528871/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:873223/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:281811/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1649366/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:354241/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1523835/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1478647/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1442190/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1440747/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1399884/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:455994/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1313975/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:538284/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:756028/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:776582/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1159122/60‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1110850/60‑1 (MQ=255)     cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcac                                      >  1:2024206/1‑60 (MQ=255)     cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcac                                      >  1:2429701/1‑60 (MQ=255)     cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcac                                      >  1:2409012/1‑60 (MQ=255)     cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcac                                      >  1:2127088/1‑60 (MQ=255)     cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcac                                      >  1:1901936/1‑60 (MQ=255)     cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcac                                      >  1:1636955/1‑60 (MQ=255)     cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcac                                      >  1:96141/1‑60 (MQ=255)     cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcac                                      >  1:1277536/1‑60 (MQ=255)     cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcac                                      >  1:1274927/1‑60 (MQ=255)     cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcac                                      >  1:1188130/1‑60 (MQ=255)         ttATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                          <  1:1701480/52‑1 (MQ=255)                              tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTaa        >  1:2352387/1‑65 (MQ=255)                              tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTaa        >  1:1955536/1‑65 (MQ=255)                              tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTaa        >  1:794666/1‑65 (MQ=255)                              tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTaa        >  1:645395/1‑65 (MQ=255)                              tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTaa        >  1:696825/1‑65 (MQ=255)                              tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTaa        >  1:791745/1‑65 (MQ=255)                              tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTACTACGGGATCCGCTCGCTTTaa        >  1:1117443/1‑65 (MQ=255)                                     cGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAgg  <  1:557153/64‑1 (MQ=255)                                     cGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAgg  <  1:2484077/64‑1 (MQ=255)                                     cGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAgg  <  1:2167617/64‑1 (MQ=255)                                     cGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAgg  <  1:1823883/64‑1 (MQ=255)                                     cGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAgg  <  1:1320185/64‑1 (MQ=255)                                              |                                                       TGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGG  >  W3110S.gb/1207021‑1207120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑