Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,249,660	A→C	Y344* (TAT→TAG)	dhaH ←	fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,249,660	0	A	C	100.0%	20.2 / NA	7	Y344* (TAT→TAG)	dhaH	fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (0/7);  total (0/7)

CATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAA  >  W3110S.gb/1249605‑1249675                                                        |                catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGcc        <  1:992868/65‑1 (MQ=255)       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa  <  1:1011239/65‑1 (MQ=255)       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa  <  1:1066123/65‑1 (MQ=255)       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa  <  1:2412037/65‑1 (MQ=255)       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa  <  1:2461312/65‑1 (MQ=255)       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa  <  1:2504306/65‑1 (MQ=255)       ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa  <  1:409480/65‑1 (MQ=255)                                                        |                CATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAA  >  W3110S.gb/1249605‑1249675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑