Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,274,654	T→C	S198P (TCA→CCA)	chaC →	regulatory protein for cation transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,274,654	0	T	C	100.0%	112.5 / NA	31	S198P (TCA→CCA)	chaC	regulatory protein for cation transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/31);  total (0/31)

TTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCA  >  W3110S.gb/1274603‑1274688                                                    |                                   tttaTTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:1792543/62‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:112408/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:725290/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:620318/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:450468/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:346353/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:2410624/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:2368770/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:2287734/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:225374/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:2218124/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:2149560/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:2099035/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:2079687/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:2038640/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:1004658/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:1646639/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:1590215/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:1575499/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:1197262/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:1462966/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:1408650/65‑1 (MQ=255) ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:1319919/65‑1 (MQ=255)  tGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:82794/64‑1 (MQ=255)  tGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGa                       <  1:207589/64‑1 (MQ=255)                      ccGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCAAACTGGGAATGCa  <  1:1520909/65‑1 (MQ=255)                      ccGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCAAACTGGGAATGCa  <  1:1961735/65‑1 (MQ=255)                      ccGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCAAACTGGGAATGCa  <  1:775669/65‑1 (MQ=255)                      ccGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCAAACTGGGAATGCa  <  1:2052448/65‑1 (MQ=255)                       cGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCAAACTGGGAATGCa  <  1:359098/64‑1 (MQ=255)                       cGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCAAACTGGGAATGCa  <  1:629504/64‑1 (MQ=255)                                                    |                                   TTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCA  >  W3110S.gb/1274603‑1274688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑