BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,275,296	A→G	intergenic (‑120/‑206)	ychN ← / → ychP	conserved hypothetical protein/predicted invasin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,275,296	0	A	G	100.0%	213.5 / NA	57	intergenic (‑120/‑206)	ychN/ychP	conserved hypothetical protein/predicted invasin
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/39); total (18/39)

GCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTT  >  W3110S.gb/1275234‑1275333

gCGTCTGACCAATCGGTCACATTTTTTAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1023997/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:643912/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:782517/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:176666/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:2432736/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1847150/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:762022/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:684158/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1945504/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:346870/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:245604/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:2136897/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:2284966/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1015540/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:2241010/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:495060/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:2284496/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1556345/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1372427/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1369540/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:814071/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:876256/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1261284/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1195207/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:944120/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:966688/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1063828/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1049731/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1031033/65‑1 (MQ=255)

gCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1017751/65‑1 (MQ=255)

 cGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGt                                     <  1:1046804/64‑1 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTg                                   >  1:1784326/1‑46 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTc                   >  1:506381/1‑62 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:390752/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:679496/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:2212985/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:325443/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:791851/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:282322/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:2505259/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:1118540/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:1310546/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:1731607/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:1859919/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:1905118/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:2074204/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:2143357/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:2267230/1‑63 (MQ=255)

                     tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCt                  >  1:2543900/1‑63 (MQ=255)

                                    cTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGtttt  <  1:2053333/64‑1 (MQ=255)

                                    cTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGtttt  <  1:2323924/64‑1 (MQ=255)

                                    cTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGtttt  <  1:1765632/64‑1 (MQ=255)

                                    cTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGtttt  <  1:2376142/64‑1 (MQ=255)

                                    cTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGtttt  <  1:2449375/64‑1 (MQ=255)

                                    cTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGtttt  <  1:1309115/64‑1 (MQ=255)

                                    cTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGtttt  <  1:1088653/64‑1 (MQ=255)

                                    cTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGtttt  <  1:992888/64‑1 (MQ=255)

GCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTT  >  W3110S.gb/1275234‑1275333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`